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pegfp c1 er alpha  (Addgene inc)


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    Structured Review

    Addgene inc pegfp c1 er alpha
    Pegfp C1 Er Alpha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1/pEGFP-C1-ER+alpha+(Plasmid+%2328230)/bio_rxiv__64898__2026__10__12__758457-75-7-9
    Average 93 stars, based on 49 article reviews
    pegfp c1 er alpha - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: The E3 ligase RNF32 controls the IκB kinase complex and NF-κB signaling in intestinal stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples A-FLX Animal Tissue FFPE BALB/C Mouse Multi Tissue Acepix Biosciences Cat# 7010-8020 Chemicals, Peptides, and Recombinant Proteins Alcian blue solution, pH2.5 Wako Cat# 015-13805 Proteasome Inhibitor MG132 PeptaNova GmbH Cat# 3175-V Lipofectamine RNAiMAX Thermo Fisher Scientific Cat# 13778100 TransIT-X2 transfection reagent Mirus Bio MIR Cat# 6003 Polyethylenimine Polysciences Cat# 23966 Polybrene Merck Cat# H9268 Puromycin dihydrochloride Merck Cat# 540411 Poly-L-lysine Merck Cat# P4707 MLN-4924 Selleck Chemical Cat# S7109 Cycloheximide Merck Cat# C4859 Thapsigargin Tocris Cat# 1138 Ionomycin Merck Cat# I0634 Phorbol 12-myristate 13-acetate Merck Cat# P8139 TNFα Peprotech Cat# 200-01B LPS Thermo Fisher Scientific Cat# 00-4976-93 W-7 hydrochloride MedChemExpress Cat# HY-100912 Calmidazolium MedChemExpress Cat# HY-103319 BioTracker 609 Red Ca2+ AM Dye Merck Cat# SCT021 FLAG peptide Merck Cat# 4799 RNF32 peptide GenScript Inc. N/A Calmodulin-Sepharose 4B beads Cytiva Cat# 17052901 Ubiquitin-K0 human Merck Cat# SRP6174 Ubiquitin-K0 human R&D Systems products Cat# UM-NOK Ubiquitin, Methylated, Human Merck Cat# 662065 Y-27632 Wako Cat# 030-24021 SB431542 Wako Cat# 192-16541 EDTA 2Na Nacalai Tesque Inc. Cat# 15130-95 BSA SIGMA Cat# A4503-50G Azoxymethane MP Biomedicals Cat# 183971 Dextran sulfate sodium MP Biomedicals Cat# 160110 Critical Commercial Assays QuickChange Site-Directed Mutagenesis Kit Stratagene Cat# 200518 Dual-Luciferase Reporter Assay System Promega Cat# E1910 RNAscope Assay ACDBio N/A RNAscope Hydrogen Peroxide solution ACDBio Cat# 322335 1X RNAscope Target retrieval solution ACDBio Cat# 322000 RNAscope Protease Plus ACDBio Cat# 322331 RNAscope Probe RNF32 ACDBio Cat# 890381 RNAscope Probe Lgr5 ACDBio Cat# 312171 RNAscope probe DapB ACDBio Cat# 310043 RNAscope 2.5 HD assay-BROWN kit ACDBio Cat# 322310 RNAscope 2.5 HD assay-RED kit ACDBio Cat# 322350 Histofine SAB-PO (M) kit Nichirei Biosciences Inc. Cat# 414314 Histofine DAB Substrate kit Nichirei Biosciences Inc. Cat# 425011 TNT Quick Coupled Transcription/Translation System Promega Cat# L1170 (Continued on next page) e2 Molecular Cell 85, 1–14.e1–e9, November 20, 2025

    Generated:

    Article Title: CARM1-mediated methylation controls interactions of ALIX with key partners important for cytokinesis
    Article Snippet: Flag-PRMT3 was from Addgene (#164695). .. Flag-PRMT2 was generated from pGex-6P-1-GST-PRMT2 (Addgene, #34650). pEGFP-C1 was a kind gift from Ludger Johannes (Institut Curie). ..

    Clone Assay:

    Article Title: The Desmoglein 2 interactome in primary neonatal cardiomyocytes
    Article Snippet: Human Desmoplakin-GFP was a gift from Kathleen Green (Addgene plasmid # 32227 ; http://n2t.net/addgene:32227 ; RRID:Addgene_32227). .. Human PKP2a was cloned from Plakophilin 2a-Flag (a gift from Kathleen Green, Addgene plasmid # 32230 ; http://n2t.net/addgene:32230 ; RRID:Addgene_32230) into pEGFP-C1. ..

    Article Title: CBFβ-SMMHC–driven leukemogenesis requires enhanced RUNX1-DNA binding affinity in mice
    Article Snippet: .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene). .. CBFB-MYH11, CBFB-MYH11-ΔHABD and CBFB-MYH11-mDE cDNA sequences were cloned into mCherry- 29 C1 vector (Addgene) respectively.

    Article Title: CBF β -SMMHC–driven leukemogenesis requires enhanced RUNX1-DNA binding affinity in mice
    Article Snippet: Eight- to 24-week-old mice and their littermate controls were injected intraperitoneally with 250 μg of pIpC (InvivoGen) every other day for 3 doses to induce the expression of Cbfb-MYH11 or Cbfb-MYH11 -ΔHABD. .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene). .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene).

    Plasmid Preparation:

    Article Title: The Desmoglein 2 interactome in primary neonatal cardiomyocytes
    Article Snippet: Human Desmoplakin-GFP was a gift from Kathleen Green (Addgene plasmid # 32227 ; http://n2t.net/addgene:32227 ; RRID:Addgene_32227). .. Human PKP2a was cloned from Plakophilin 2a-Flag (a gift from Kathleen Green, Addgene plasmid # 32230 ; http://n2t.net/addgene:32230 ; RRID:Addgene_32230) into pEGFP-C1. ..

    Sequencing:

    Article Title: CBFβ-SMMHC–driven leukemogenesis requires enhanced RUNX1-DNA binding affinity in mice
    Article Snippet: .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene). .. CBFB-MYH11, CBFB-MYH11-ΔHABD and CBFB-MYH11-mDE cDNA sequences were cloned into mCherry- 29 C1 vector (Addgene) respectively.

    Article Title: CBF β -SMMHC–driven leukemogenesis requires enhanced RUNX1-DNA binding affinity in mice
    Article Snippet: Eight- to 24-week-old mice and their littermate controls were injected intraperitoneally with 250 μg of pIpC (InvivoGen) every other day for 3 doses to induce the expression of Cbfb-MYH11 or Cbfb-MYH11 -ΔHABD. .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene). .. Runx1b cDNA sequence was cloned into pEGFP-C1 (Addgene).

    Transfection:

    Article Title: Zika virus capsid protein C (ZIKV-C) interactors network mapped by proteomic analysis of human neural stem cells expressing FLAG-tagged C protein
    Article Snippet: .. Two plasmids were used for the transfection of cultured NSCs: pLV_Zika_Cv_Flag was a gift from Vaithi Arumugaswami (Addgene) and pEGFP-C1 (Clontech). ..

    Cell Culture:

    Article Title: Zika virus capsid protein C (ZIKV-C) interactors network mapped by proteomic analysis of human neural stem cells expressing FLAG-tagged C protein
    Article Snippet: .. Two plasmids were used for the transfection of cultured NSCs: pLV_Zika_Cv_Flag was a gift from Vaithi Arumugaswami (Addgene) and pEGFP-C1 (Clontech). ..



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    Addgene inc plasmid encoding erα
    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and <t>ERα</t> (red dots) in MCF-7 cells and <t>transfected</t> <t>HEK293T</t> cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.
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    Plasmids.

    Journal: Autophagy

    Article Title: CHCHD2 and CHCHD10 promoted autophagic clearance of protein aggregates via GABARAPs

    doi: 10.1080/15548627.2026.2678427

    Figure Lengend Snippet: Plasmids.

    Article Snippet: pEGFP-C1-GABARAP , Addgene , 87871 (Eiki Kominami Lab).

    Techniques: Plasmid Preparation

    In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Journal: bioRxiv

    Article Title: Antidepressants interact with sex steroid receptors and their intracellular signaling components

    doi: 10.64898/2026.03.17.712321

    Figure Lengend Snippet: In situ PLA demonstrates close proximity between biotinylated fluoxetine as well as HNK and ERα (red dots) in MCF-7 cells and transfected HEK293T cells (magenta), while no PLA signal was detected in HEK293T cells not expressing ERα. ERα-transfected cells are tagged with GFP and are represented in magenta. Blue represents DAPI staining. Arrows point to the PLA signal.

    Article Snippet: A group of HEK293T cells were transfected with a plasmid encoding ERα (pEGFP-C1-ER alpha, Addgene, Plasmid #28230) using Lipofectamine 2000 (Thermo Fisher Scientific).

    Techniques: In Situ, Transfection, Expressing, Staining